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SARS-like cluster of circulating bat coronavirus pose threat for human emergence

SARS-like cluster of circulating bat coronavirus pose threat for human emergence

Vineet D. Menachery1, Boyd L. Yount Jr1, Kari Debbink1,2, Sudhakar Agnihothram3, Lisa E. Gralinski1, Jessica A. Plante1, Rachel L. Graham1, Trevor Scobey1, Xing-Yi Ge8, Eric F. Donaldson1, Scott H. Randell4,5, Antonio Lanzavecchia6, Wayne A. Marasco7, Zhengli-Li Shi8, and Ralph S. Baric1,2 1Departments of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 2Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 3National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR, USA 4Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 5Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 6 Institute for Research in Biomedicine, Bellinzona, Switzerland Institute of Microbiology, ETH Zurich, Zurich, Switzerland 7Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute; Department of Medicine, Harvard Medical School, Boston Massachusetts, USA 8Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China Abstract The emergence of Severe Acute Respiratory Syndrome

Coronavirus (SARS-CoV) and Middle East Respiratory Syndrome (MERS)-CoV underscores the threat of cross-species transmission events leading to outbreaks in humans. In this study, we examine the disease potential for SARSlike CoVs currently circulating in Chinese horseshoe bat populations. Utilizing the SARS-CoV infectious clone, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse adapted SARS-CoV backbone. The results indicate that group 2b viruses encoding the SHC014 spike in a wild type backbone can efficiently utilize multiple ACE2 receptor orthologs, replicate efficiently in primary human airway cells, and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis. Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from CoVs utilizing the novel spike protein. Importantly, based on these findings, we synthetically rederived an infectious full length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Together, the work highlights a continued risk of SARS-CoV reemergence from viruses currently circulating in bat populations.

https://www.med.unc.edu/orfeome/files/2018/03/a-sars-like-cluster-of-circulating-bat-coronaviruses-shows-potential-for-human-emergence.pdf?fbclid=IwAR3c_PY5RbTdLHuYxRZUcTPQHdQjFzy8JN6TUsygDRq0DkDEH_GrPKzuX-c

Профессор Ральф Барик - глава кафедры эпидемиологии Университета Северной Каролины в Чапел-Хилл, с соавт. создатель коронавируса SARS-cov2.

Вот их статья 2015 года: https://www.med.unc.edu/…/a-sars-like-cluster-of-circulatin…

Они вывели очень похожий на Covid19 вирус чтоб доказать, что вирусы летучих мышей могут быть очень опасны.

В статье они взяли за основу вирус атипичной пневмонии и "надели" на него шипы от вируса летучих мышей из Уханя (там китаец Ченгли Ли Ши соавтор, он и передал материал...). Затем пассировали полученную химеру на клетках верхних дыхательн путей человека, чтоб он лучше приспособился.

Получился патогенный вирус с высокой инфекционностью для человека и устойчивый к известным препаратам и сывороткам.

Кто знает, сколько подобных вирусов они сконструировали, но не опубликовали.
***

Итого, текущий вирус действительно очень опасен и противоэпидемические мероприятия оправданы.

ЗЫ: при репосте копируйте текст, иначе публикуется только статья на английском.

Отсюда

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